: 5683

MesenCult™-ACF Culture Kit

: 05449

Defined, animal component-free culture kit for human mesenchymal stem cells

:

    Overview
    The MesenCult™-ACF Culture Kit is a standardized, animal component-free and serum-free system for the isolation and culture of human mesenchymal stem cells (MSCs). It consists of MesenCult™-ACF Medium (Catalog #05440) and MesenCult™-ACF Attachment Substrate (Catalog #05444) and is optimized for the expansion of MSCs in vitro as well as their enumeration using the colony-forming unit-fibroblast (CFU-F) assay. This product supports the long-term growth of human bone marrow- and adipose tissue-derived MSCs as cells maintain robust multi-lineage differentiation potential. MesenCult™-ACF Medium must be used in conjunction with the MesenCult™-ACF Attachment Substrate and the MesenCult™-ACF Dissociation Kit (Catalog #05426). Components of the MesenCult™-ACF Attachment Substrate and the MesenCult™-ACF Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Medium. MesenCult™-ACF Medium must be supplemented with L-glutamine.
    Advantages:
    • Defined, animal component-free culture system 
    • Optimized for MSC isolation from primary bone marrow and adipose tissue 
    • Superior cell expansion compared to serum-containing medium 
    • MSC cultures display robust self-renewal and differentiation potential after long-term passaging 
    • No adaptation required when transitioning MSCs from serum-containing medium
    Components:

    • MesenCult™-ACF Culture Kit (Catalog #05449)
      • MesenCult™-ACF Basal Medium, 400 mL (Catalog #05451)
      • MesenCult™-ACF 5X Supplement, 100 mL (Catalog #05452)
      • MesenCult™-ACF Attachment Substrate, 1 mL (Catalog #05444)
    Subtype:
    Specialized Media
    Cell Type:
    Mesenchymal Stem and Progenitor Cells
    Species:
    Human
    Application:
    Cell Culture; Colony Assay; Expansion
    Brand:
    MesenCult
    Area of Interest:
    Stem Cell Biology
    Formulation:
    Animal Component-Free; Defined; Serum-Free