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NEW PRODUCT

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9,410,795

: 12345

LUNA-II™ Automated Cell Counter

: luna-ii

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LUNA-II™ Automated Cell Counter
LUNA-II™ Automated Cell Counter
Fully automated cell counting and cell viability analysisAutofocus technology for fast and reliable cell countingTrypan blue stained cell counting or non-stained cell counting methods Various histograms and cell-size gatingUnmatched counting accuracy and speedBuilt-in computer and printerThe most affordable running cost currently on the market
INTERACTIVE SOFTWARE INTERFACE

Options for staining methods

The LUNA-II™ automated cell counter can count samples with or without trypan blue dye. The selection of trypan blue presence
or absence can easily be chosen in the settings menu.

Cell size histogram and cell size based gating

After counting, cells can be gated based on their size information provided by the LUNA-II™. The specific size of cell populations
Can easily be included or excluded on histograms.

Cell cluster map

Counted cells can be sorted with cell cluster map, into single cells, doublets, or triplets.
The cell cluster map can be used to monitor changes in culture conditions or cell isolation/preparation protocols.

Review and Re-analysis option

The LUNA-II™ automated cell counter provides a powerful review option.
A separate PC unnecessary. Users can open cell images directly on the LUNA-II™ automated cell counter
to check previous results.

Various file save options

The LUNA-II™ can store up to 1,000 counts onboard. After counting, the result is automatically saved in the memory, and previous count results can be exported via USB as a .CSV file for further analysis. The results and image data can be saved in three different file formats such as TIF, annotated TIF, or a PDF report file. The PDF report file contains all the data generated during/after the cell counting, i.e., the protocol used, cell counting results, date, raw/analyzed cell image(s), and histograms are included in the PDF report file.

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NEW AUTOFOCUSING TECHNOLOGY

The LUNA-II™ automated cell counter has integrated a novel focusing mechanism based on liquid lens technology.
The liquid lens does not use any mechanical moving parts to change the Z stage. Instead, the Z position of the sample is rapidly
obtained by the application of a small voltage to the liquid lens. The elimination of moving parts removes noise and significantly
reduces the need for servicing. Even after multi-million cycles of focusing, the liquid lens does not require repair or service.
In combination with this novel focusing mechanism, the LUNA-II™ has integrated a new autofocusing algorithm optimized for
cell counting.

liquid lens autofocusing mechanism

CELL COUNTING ACCURACY : DE-CLUSTERING

The LUNA-II™ automated cell counting algorithm has been recognized to have the best-in-class cell counting accuracy.
The LUNA™ software has exceptional accuracy in cell de-clustering and can successfully count clumpy cells. The LUNA-II™ automated
cell counter has inherited this well-known performance aspect of the LUNA™ software. Clumpy cells are de-clustered quickly
and automatically, then counted as individual cells.

REDUCED RUNNING COST

Automated cell counters utilize disposable counting slides to eliminate washing steps of manual cell counting with the glass
hemocytometer. Although disposable cell counting slides have several advantages, the increased running cost has been a substantial
concern. Logos Biosystems developed a patented T-BOND technology to manufacture precision cell counting slides more efficiently.
Therefore, the unit price of counting slides became much more affordable, providing significant cost savings.

BUILT – IN PRINTER

A thermal printer is integrated in the LUNA-II™ automated cell counter, and the counting results can be printed immediately for
record keeping purposes. Because the printer is already integrated in the LUNA-II™ automated cell counter, an additional cable
connection is not required.

~~The LUNA™ family helps scientists worldwide obtain accurate and meaningful cell count and viability data.
Browse through some of the studies that the LUNA™ family has been involved in below.
LUNA™ automated cell counter
LUNA-FL™ dual fluorescence cell counter
LUNA-II™ automated cell counter
LUNA-STEM™ SVF automated cell counter

Citations of LUNA-FL™ Automated Cell Counter

1. Ramirez KG, Shea DT, McKim DB, Reader BF, Sheridan JF. Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Brain BehavImmun 2015;pii:S0889-S1591.

2. Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I. β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells. Exp Mol Med 2015;47:e143.

3. Hong D, Lee G, Jung NC, Jeon M. Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images. Biol Proced Online 2013;15:13.

Citations of LUNA™ Automated Cell Counter

1. Yuasa M, Yamada T, Taniyama T, Masaoka T, Xuetao W, Yoshii T, Horie M, Yasuda H, Uemura T, Okawa A, Sotome S. Dexamethasone enhances osteogenic differentiation of bone marrow- and muscle-derived stromal cells and augments ectopic bone formation induced by bome morphogenetic protein-2. PLoS One 2015;10:e0116462.

2. Renner SP, Strissel PL, Beckmann MW, Lermann J, Burghaus S, Hackl J, Fasching PA, Strick R. Inhibition of adhesion, proliferation and invasion of primary endometriosis, endometrial stromal and ovarian carcinoma cells by a non-hyaluronan adhesion barrier gel. BioMed Res Int. 2015;2015:450468

3. Kim EC, Kim HJ, Kim TJ. Water extract of Cinnamomum cassia suppresses angiogenesis through inhibition of VEGF receptor 2 phosphorylation. BiosciBiotechnol Biochem 2015; DOI:10.1080/09168451.2014.993917.

4. Bahn YJ, Lee KP, Lee SM, Choi JY, Seo YS, Kwon KS. Nucleoredoxin promotes adipogenic differentiation through regulation of Wnt/β-catenin signaling. J Lipid Res 2015;56:294-303.

5. Lazra Y, Falach A, Frenkel L, Rozenberg K, Sampson SR, Rosenzweig T. Autocrine/paracrine function of globular adiponectin: inhibition of lipid metabolism and inflammatory response in 3T3-L1 adipocytes. J Cell Biochem 2015;DOI: 10.1002/jcb.25031.

6. Nakamura T, Shinriki S, Jono H, Ueda M, Nagata M, Guo J, Hayashi M, Yoshida R, Ota T, Ota K, Kawahara K, Nakagawa Y, Yamshita S, Nakayama H, Hiraki A, Shinohara M, Ando Y. Osteopontin-integrin αvβ3 axis is crucial for 5-fluorouracil resistance in oral squamous cell carcinoma. FEBS Lett 2015;589:231-239.

7. Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I. β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells. Exp Mol Med 2015;47:e143.

8. Yi YW, Oh S. Comparative analysis of NRF2-responsive gene expression in AsPC-1 pancreatic cancer cell line. Genes Genom 2015;37:97-109.

9. Sun T, Tsan WM, Park WT, Merugu S. Surface characteristics, biodegradability and biocompatibility of porous silicon for microfabricated neural electrode. J NanosciNanotechnol 2015;15:2821-2828.

10. Kang HJ, Yi YW, Hong YB, Kim HJ, Jang YJ, Seong YS, Bae I. HER2 confers drug resistance of human cancer cells through activation of NRF2 by direct interaction. Sci Rep 2014;4:7201.

11. Masuda T, Yilling S, Eui SW, Niimi M, Yusa A, Nakanishi H, Ara F. Cell isolation system for rare circulating tumor cell. 2014 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2014). 2014;DOI: 10.1109/IROS.2014.6943230.

12. Cho YS, Hong MW, Kim SY, Lee SJ, Lee JH, Kim YY, Cho YS. Fabrication of dual-pore scaffolds using SLUP (salt leaching using powder) and WNM (wire-network molding) techniques. Mat Sci Eng C 2014;45:546-555.

13. Adimule V, Medapa S, Jagadeesha AH, Kumar LS, Rao PK. Synthesis, characterization and cytotoxic evaluation of novel derivatives of 1,3,4-oxadiazole containing 5-phenyl thiophene moiety. IOSR J Pharm Biol Sci 2014;9:42-48.

14. Bieler J, Cannavo R, Gustafson K, Gobet C, Gatfield D, Naef F. Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells. Mol Syst Biol 2014;10:739.

15. Yalcin I, Horakova J, Mikes P, Sadikoglu TG, Domin R, Lukas D. Design of polycaprolactone vascular grafts. J Ind Text 2014; doi: 10.1177/1528083714540701.

16. Schnell A, Chappuis S, Schmutz I, Brai E, Ripperger J, Schaad O, Welzl H, Descombes P, Alberi L, Albrecht U. The nuclear receptor REV-ERBα regulates Fabp7 and modulates adult hippocampal neurogenesis. PLoS One 2014;9:e99883.

17. Yamanaka S, Yokote S, Yamada A, Katsuoka Y, Izuhara L, Shimada Y, Omura N, Okano HJ, Ohki T, Yokko T. Adipose tissue-derived stem cells in long-term dialysis patients display downregulation of PCAF expression and poor angiogenesis activation. PLoS One 2014;9:e102311.

18. Kim SY, Lee DH, Song X, Bartlett DL, Kwon YT, Lee YJ. Role of Bcl-xL/Beclin-1 in synergistic apoptotic effects of secretory TRAIL-armed adenovirus in combination with mitomycin C and hyperthermia on colon cancer cells. Apoptosis 2014;19:1603-1615.

19. Arasada RR, Amann JM, Rahman MA, Huppert SS, Carbone DP. EGFR blockade enriches for lung cancer stem-like cells through Notch3-dependent signaling. Cancer Res 2014;74:5572-5584.

20. Lui YLE, Tan TL, Woo WH, Timms P, Hafner LM, Tan KH, Tan EL. Enterovirus71 (EV71) utilize host microRNAs to mediate host immune system enhancing survival during infection. PLoS One 2014;9:e102997.

21. Lee JW, Kwon JS, Om JY, Kim YH, Choi EH, Kim KM, Kim KN. Cell immobilization on polymer by air atmospheric pressure plasma jet treatment. Jpn J ApplPhys 2014;53:086202.

22. Patel MN, Lakkadwala S, Majrad MS, Injeti ER, Gollmer SM, Shah ZA, Boddu SHS, Nesamony J. Characterization and evaluation of 5-fluorouracil-loaded solid lipid nanoparticles prepared via a temperature-modulated solidification technique. AAPS PharmSciTech 2014;15:1498-1508.

23. Song B, Kim B, Choi SH, Song KY, Chung YG. Lee YS, Park G. Mesenchymal stromal cells promote tumor progression in fibrosarcoma and gastric cancer cells. Kor J Pathol 2014;48:217-224.

24. Hou SJ, Yi YW, Kang HJ, Zhang L, Kim HJ, Kong Y, Liu Y, Wang K, Kong HS, Grindrod S, Bae I, Brown ML. Novel carbazole inhibits phospho-STAT3 through induction of protein tyrosine phosphatase PTPN6. J Med Chem 2014;57:6342-6353.

25. Hassan MI, Sun T, Sultana N. Fabrication of nanohydroxyapatite/poly(caprolactone) composite microfibers using electrospinning technique for tissue engineering applications. J Nanomaterials 2014;2014:209049.

26. Samara A, Galbiati M, Luciani P, Deledda C, Messi E, Peri A, Maggi R. Altered expression of 3-betahydroxysterol delta-24-reductase/selective Alzheimer’s disease indicator-1 gene in Huntington’s disease models. J Endocrinol Invest 2014;37:729-737.

27. Jo DH, Kim JH, Son JG, Piao Y, Lee TG, Kim JH. Inhibitory activity of gold and silica nanspheres to vascular endothelial growth factor (VEGF)-mediated angiogenesis is determined by their sizes. Nano Res 2014;7:844-852.

28. Kwon JS, Kim KM, Kim KN. A comparative study of three cytotoxicity test methods for nanomaterials using sodium lauryl sulfate. J NanosciNanotechnol 2014;14:8043-8047.

29. Jeong SH, Kim HK, Song IS, Lee SJ, Ko KS, Rhee BD, Kim N, Mishchenko NP, Fedoryev SA, Stonik VA, Han J. Echinochrome A protects mitochondrial function in cardiomyocytes against cardiotoxic drugs. Mar Drugs 2014;12:2922-2936.

30. Duong HQ, Yi YW, Kang HJ, Hong YB, Tang W, Wang A, Seong YS, Bae I. Inhibition of NRF2 by PIK-75 augments sensitivity of pancreatic cancer cells to gemcitabine. Int J Oncol 2014;44:959-969.

31. Lee JH, Park JW. Attenuated mitochondrial NDAP+-dependent isocitrate dehydrogenase activity induces apoptosis and hypertrophy of H9c2 cardiomyocytes. Biochimie 2014;99:110-118.

32. Lakkadwala S, Nguyen S, Lawrence J, Nauli SM, Nesamony J. Physico-chemical characterization, cytotoxic activity, and biocompatibility studies of tamoxifen-loaded solid lipid nanoparticles prepared via a temperature-modulated solidification method. J Microencapsul 2014;31:590-599.

33. Yusa A, Toneri M, Masuda T, Ito S, Yamamoto S, Okochi M, Kondo N, Iwata H, Yatabe Y, Ichinosawa Y, Kinuta S, Kondo E, Honda H, Arai F, Nakanishi H. Development of a new rapid isolation device for circulating tumor cells (CTCs) using 3D palladium filter and its application for genetic analysis. PLoS One 2014;9:e88821.

34. Sun T, Tsang WM, Park WT. Drug release from porous silicon for stable neural interface. Appl Surface Sci 2014;292:843-851.

35. Sun T, Khan TH, Sultana N. Fabrication and in vitro evaluation of nanosized hydroxyapatite/chitosan-based tissue engineering scaffolds. J Nanomaterials 2014;2014:194680.

36. Lee LT, Ng SY, Chu JY, Sekar R, Harikumar KG, Miller LJ, Chow BK. Transmembrane peptides as unique tools to demonstrate the in vivo action of a cross-class GPCR heterocomplex. FASEB J 2014;28:2632-2644.

37. Hong MH, Kim SM, Om JY, Kwon N. Lee YK. Seeding cells on calcium phosphate scaffolds using hydrogel enhanced osteoblst proliferation and differentiation. Annals Biomed Eng 2014;42:1424-1435.

38. Rodrigues HF, Mello FM, Branguinho LC, Zufelato N, Silverira-Lacerda EP, Bakuzis AF. Real-time infrared thermography detection of magnetic nanoparticle hyperthermia in a murine model under a non-uniform field configuration. Int J Hyperthermia 2013;29:752-767.

39. Yusa A, Matsumoto Y, Terazawa K, Masuda T, Arai F, Nakanishi H. Isolation of living circulating tumor cells (CTCs) from peripheral blood using size-based device and its application to CTC technology in mice. IEEE 2013 International Symposium on Micro-NanoMechatronics and Human Science (MHS). 2013;DOI: 10.1109/MHS.2013.6710455.

40. Liu YLE, Timms P, Hafner LM, Tan TL, Tan KH, Tan EL. Characterization of enterovirus 71 replication kinetics in human colorectal cell line, HT29. SpringerPlus 2013;2:267.

41. Montero D, Tachibana C, Winther JR, Appenzeller-Herzog C. Intracellular glutathione pools are heterogeneously concentrated. Redox Biol 2013;1:508-513.

42. Leung TH, Wong SC, Chan KK, Chan DW, Cheung AN, Ngan HY. The interaction between C35 and ΔNp73 promotes chemo-resistance in ovarian cancer cells. Br J Cancer 2013;109:965-975.

43. Duong HQ, Hong YB, Kim JS, Lee HS, Yi YW, Kim YJ, Wang A, Zhao W, Cho CH, Seong YS, Bae I. Inhibition of checkpoint kinase 2 (CHK2) enhances sensitivity of pancreatic adenocarcinoma cells to gemcitabine. J Cell Mol Med 2013;10:1261-1270.

44. Yi YW, Kang HJ, Kim HJ, Kong Y, Brown ML, Bae I. Targeting mutant p53 by a SIRT1 activator YK-3-237 inhibits the proliferation of triple-negative breast cancer cells. Oncotarget 2013;4:984-994.

45. Yi YW, Hong W, Kang HJ, Kim HJ, Zhao W, Wang A, Seong YS, Bae I. Inhibition of the PI3K/AKT pathway potentiates cytotoxicity of inhibitors for EGFR kinase in triple-negative breast cancer cells. J Cell Mol Med 2013;17:648-656.

46. Sajay BNG, Chan CP, Ahmad H, Chung WC, Puiu PD, Rahman ARA. Towards an optimal and unbiased approach for tumor cell isolation. Biomed Microdevices 2013;15:699-709.

47. Kwon JS, Kim YH, Choi EH, Kim KN. The effects of non-thermal atmospheric pressure plasma jet on attachment of osteoblast. CurrApplPhys 2013;13:S42-S47.

48. Nam JO, Son HN, Jun E, Cha K, Lee BH, Park RW, Kim IS. FAS1 domain protein inhibits VEGF165-induced angiogenesis by targeting the interaction between VEGFR-2 and αvβ3 integrin. Mol Cancer Res 2012;10:1010-1020.

49. Duong HQ, Hwang JS, Kim HJ, Seong YS, Bae I. BML-275, an AMPK inhibitor, induces DNA damage, G2/M arrest and apoptosis in human pancreatic cancer cells. Int J Oncol 2012;41:2227-2236.

50. Duoung HQ, Hwang JS, Kim HJ, Kang HJ, Seong YS, Bae I. Aldehyde dehydrogenase 1A1 confers intrinsic and acquired resistance to gemcitabine in human pancreatic adenocarcinoma MIA PaCa-2 cells. Int J Oncol 2012;41:855-861.

51. Kang HJ, Yi YW, Kim HJ, Hong YB, Seong YS, Bae I. BRCA1 negatively regulates IGF-1 expression through an estrogen-responsive element-like site. Cell Death Dis 2012;3:e336.

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